The prestige of avoiding cross contamination cannot be overemphasized. The crime step investigator performing the collection must ensure tools are clean or sterilized and also that gloves are readjusted between managing each sample.

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Crime scene collection techniques differ depending upon the kind of evidence and also the substrate ~ above which that is found. The is preferable come collect proof in its initial state. If the proof is vulnerable or can quickly be lost, the entire object must be gathered and packaged, if size and also circumstances permit.

Some laboratories recommend the entry of substrate controls. Substrate controls are clean samples that the collection products or unstained portions of the material the biological evidence is deposit on. The laboratories deserve to use this to troubleshoot contamination, Polymerase Chain Reaction (PCR) inhibition, or interference v fluorescence.

The investigator have to consult the local forensic laboratory and refer come the department traditional operating procedures concerning crime scene arsenal techniques and also preservation of organic evidence.

Crime scene Collection methods For Blood & various other Body Fluids


Removal that a ar of the article containing the stain using a sterile or clean cut device.

Wet Absorption:

A sterile swab, gauze pad, or threads space slightly moistened with sterile distilled water. An initiative should be made to concentration the stain in a localized part of the swab or pad. Because that example, once using a swab, the stain should be focused on the tip. The collection medium is concentrated into the stain and allowed to wait dry. Part laboratories recommend complying with the an initial moistened swabbing through a second dry swabbing come ensure thoroughly sample collection. Both swabs are retained and submitted because that analysis.

Scraping Method:

Using a clean razor blade or scalpel, the sample is scraped into a clean piece of document that deserve to be folded and packaged in a paper envelope or other ideal packaging.

Lifting with Tape:

For dried blood stains on a non-absorbent surface, fingerprint lifting tape might be placed over the stain and also lifted off. The stain is moved to the adhesive next of the tape, which might then it is in secured top top a clear piece of acetate for submission to the laboratory.

Crime scene Collection techniques For Hair & Fibers

Visual Collection:

On part surfaces, hairs and fibers deserve to be seen v the naked eye. Making use of clean forceps and also trace paper, the sample can be removed from the surface and also placed into a clean piece of paper that deserve to be folded and packaged in a file envelope or other suitable packaging.

Tape Lifting:

Water or methanol soluble tapes are easily accessible for the collection of trace hair and also fiber evidence. The ice cream is used to the location of the suspected sample, removed, and also packaged.

Vacuuming Method:

The area wherein the suspected samples are located are vacuumed up and also caught in a filtered trap attached come the vacuum. These samples space packaged in clean trace file for entry to the laboratory. Vacuuming is the least desirable collection technique because over there is a threat of cross pollution if the equipment is not effectively cleaned between each use.

Reference Sample Crime Scene repertoire Techniques

Reference samples need to be collected from people who might be connected to the crime scene whereby DNA proof is found. Reference samples can be provided for elimination or compare analysis. For example, buccal swab samples taken from the suspect and/or victim, a known source, should be compared to organic evidence uncovered at the crime step to eliminate or place them in ~ the scene.

Buccal Swab:

Sterile swabs or various other buccal collection devices are rubbed versus the within cheek of the individual"s mouth to collect epithelial cells because that analysis.

Liquid Blood Samples:

Generally built up in violet topped vacuum tubes that contain the preservative ethylenediamine tetraacetic acid (EDTA).

Packaging & Storage:

Biological proof should it is in dried prior to packaging to minimization sample degradation. Packaging in document is preferred; however, some laboratories allow packaging in plastic if the sample is extensively dried.

Liquid samples, such as water native a toilet key or pipes, have to be effectively documented and also packaged in sterile glass or plastic containers and also refrigerated as shortly as possible.

(Information listed by the room of Justice)

Essential Reading


In a civilization heavily influenced by renowned forensic television dramas, the real-life duties and also complexities associated in crime-scene examination are frequently misrepresented and also misunderstood. An arrival to Crime-Scene Investigation, 3rd Edition is a an extensive and accurate overview of the useful application that forensic scientific research in crime step investigation. Focusing on the day-to-day aspects, this full-color text defines the methodologies and also technologies employed by crime step personnel, and also pushes the leader to identify the interrelated materials of the investigative process.

Recognizing the prestige of those who have added to and advanced the field of forensic science, the leader is introduced to CSI through a historical perspective. The updated third Edition covers materials not frequently found in many crime step texts, including moral considerations and also public misconceptions that the investigative process—the so-called “CSI Effect.” Extensive conversation of physical proof examination and analysis is provided, including the forensic potential of physics evidence, crime scene procedures, and what evidence should be documented, collected, and preserved. Real-life examples and over 250 detailed, full-color photos and figures enhance comprehension and demonstrate details strategies for method application

While the vast majority of the message is written v an American audience in mind, the author has preferred to call upon his far-ranging international experience and also include cases, information, and also photographs/figures which are exterior to the joined States.

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An introduction to Crime-Scene Investigation, 3rd Edition is one invaluable reference and also essential education resource.